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Thrust V: Addressing International Fisheries Related Issues from a Regional Perspective 20 Assistance of Capacity Building in the Region to Address

1. INTRODUCTION/BACKGROUND 1 Situation Analysis

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Appendix 6 of Annex 4 PROJECT DOCUMENT

Project id: 031301

Program Categories:

Programs under the Fisheries Consultative Group of the ASEAN-SEAFDEC Strategic Partnership (FCG/ASSP) Mechanism

Project Title: Chemical and Drug Residues in Fish and Fish Products in Southeast Asia - Biotoxin Monitoring in ASEAN Region: ASP, AZA and BTX

Program Thrust: II Total Duration: 5 yrs (2013-2017)

Lead Department: MFRD Lead Country: Singapore

Project Sponsor: Japanese Trust Fund-VI Project Partner: Post-Harvest Technology Centre, Agri-Food and Veterinary Authority of Singapore (PHTC/AVA) Proposed Budget: USD 190,000 This year budget: (2013) USD 35,000 Prepared by Neo Shan Yu, Scientist

(PHTC/AVA); Yeap Soon Eong, Chief of MFRD Programmes

Project Leader Neo Shan Yu, Scientist (PHTC/AVA)

1. INTRODUCTION/BACKGROUND

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Furthermore, the growing harvest of non-traditional shellfish (such as moon snails, whelks, barnacles, etc) may increase human health problems and management responsibilities. Member Countries have raised the need to continue with the capability building in biotoxins analyses and monitoring, with the proposed focus on other biotoxins like Amnesic Shellfish Poisoning (ASP) toxin and Azaspiracids (AZA). These 2 biotoxins were raised as part of the training needs by Member Countries during the RTC of the previous project in 2009. Brevetoxins (BTX) is also recommended to be included in the new project as ASP, AZA and BTX, along with DSP and PSP, should be regulated according to CODEX for shellfish.

1.4 Links to Regional Provisions

The project is in line with the following Resolution and Plan of Action as endorsed at the ASEAN- SEAFDEC Conference of 2011:

Resolution 21: Improve technologies and facilities to ensure fish quality assurance and safety management systems, taking into account the importance of traditional fishery products and food security requirements, and promote the development of fishery products as an alternative supplementary livelihood for fisheries communities

Plan of Action D61: Strengthen fish quality and safety management systems that support the competitive position of ASEAN fish products on world markets, including moving towards ISO/IEC 17025 accreditation of national fish inspection laboratories, strengthening capacity and acknowledging the recognized national laboratories, risk analysis and equivalence agreement such as the Mutual Recognition Agreement (MRA) and promote the implementation of the quality and safety management systems among small and medium enterprises in the ASEAN region.

Plan of Action D63: Promote and conduct training programs and develop training materials to upgrade the technical skills and competencies of personnel in the public and private sectors on fisheries post-harvest technology and food safety management system.

1.5 Links to SEAFDEC Program Thrust

Thrust II: Enhancing Capacity and Competitiveness to Facilitate International and Intra-Regional Trade 1.6 Links with other SEAFDEC’s Projects

This project is an extension of the previous Japanese Trust Fund II project (2009-2012) on biotoxins monitoring which focused Diarrhoetic Shellfish Poisoning (DSP) toxins, lipophilic toxins, Paralytic Shellfish Poisoning (PSP) toxins and Tetradotoxin (TTX).

2. PROJECT

2.1 Goal/Overall Objectives and Performance Indicators:

The goal of the project is to enhance regional capability and knowledge in biotoxins testing and monitoring, and biotoxins occurrences and incidences in fish and shellfish in the ASEAN region. The objectives of the project are to:

1) Upgrade regional laboratory capabilities and credibility for testing of Amnesic Shellfish Poisoning (ASP), Azaspiracids (AZA) and Brevetoxin (BTX) biotoxins for fish and fisheries products through conduct of a regional training course on methodologies for Member Countries and 1.5 year biotoxins monitoring survey in Member Countries;

2) Establish biotoxins monitoring programmes for ASP, AZA and BTX biotoxins in Member Countries for routine surveillance testing of fish and fisheries products especially in those countries that do not have such programmes; and

3) Improve knowledge and understanding on levels of ASP, AZA and BTX biotoxins occurrences and incidences in fish and fisheries products in the ASEAN region and facilitate exchange of information among Member Countries.

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The performance indicators of the project are the establishment of methodologies for testing of the target biotoxins and the conduct of the biotoxins monitoring survey in Member Countries.

2.2 Expected Outcomes and Outputs:

The expected outcomes of the project are enhanced laboratory capabilities and knowledge in the testing of ASP, AZA and BTX biotoxins, establishment of monitoring programmes for ASP, AZA and BTX biotoxins in Member Countries for routine surveillance and, improved knowledge and understanding on ASP, AZA and BTX biotoxins occurrences and incidences in fish and shellfish in the ASEAN region. The expected outputs are:

1) A regional training course on AZA, ASP and BTX biotoxins analyses;

2) Biotoxin monitoring surveys in Member Countries; and

3) Technical Compilation of Biotoxins (ASP, AZA and BTX) Monitoring in ASEAN region.

2.3 Project Description/Framework

The Post-Harvest Technology Centre (PHTC) of the Agri-Food and Veterinary Authority (AVA) as the Collaborating Center for MFRD programmes will be responsible for the project and will manage and coordinate all project activities. The project will be conducted for a period of 5 years from 2013-2017 and funded under the Japanese Trust Fund-VI program on a cost-sharing basis with the participating ASEAN- SEAFDEC Member Countries. The project will be implemented through the following activities:

Activity 1: Regional Technical Consultation (RTC) in 2013

A RTC will be held in Singapore to initiate the project and plan for all project activities. The RTC will discuss the details of the regional training such as the venue and key trainers, the targeted biotoxins for the survey and the key project leaders in each country.

Activity 2: Regional Training Course in Biotoxins Analyses in 2014

The proposed biotoxins for training in this course will include ASP, AZA and BTX. It will include lectures and hands-on practical sessions and will be conducted by invited Japanese expert trainers, with two participants from each member country.

Activity 3: Biotoxins Monitoring Survey in 2015 and 2016

After the training course, Member Countries are encouraged to set up the biotoxins analyses methods and use these methods for the survey. The survey scope will cover regular monitoring of the biotoxins (AZA, ASP and BTX) levels in selected shellfish species at identified site(s) over a one and a half-year period.

Biotoxins that were already covered in the previous project in 2010 (for example DSP and lipophilic toxins, TTX) can also be included in the present survey if Member Countries are interested.

Activity 4: Preparation and Publication of Technical Compilation in 2016 and 2017

A Technical Compilation of the project will be prepared and published to document the project outcomes/outputs. The Technical Compilation will include the biotoxins analyses methodologies, the biotoxins monitoring survey methodologies and the survey reports and results of participating Member Countries.

Activity 5: End-of-Project Seminar (EOP) in 2017

The EOP will be organized in the final year of the project to conclude the project and share the project results among Member Countries. The EOP will also discuss the progress achieved in the biotoxins analytical and monitoring capabilities in Member Countries over the years, the challenges faced and the plans for the future. The Technical Compilation will be discussed and finalized for publication at the EOP.

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3. PROGRESS/ACHIEVEMENTS OF ACTIVITIES IN THE YEAR 2013 3.1 Activities Achievements in the Year 2013

Achievements based on Activities Duration Remarks Activity 1: Regional Technical Consultation (RTC)

- The RTC was conducted successfully in Singapore. It was attended by all 10 ASEAN Member Countries, SEAFDEC Secretariat and project resource person, Dr. Toshiyuki Suzuki, from the National Research Institute of Fisheries Science, Japan.

- The RTC met its objectives to deliberate and discuss on the project activities and the project schedule as well as details of the regional training course on analysis of the biotoxins and the monitoring survey to be carried out in participating countries. The training needs in Member Countries, content, venue and trainers for the training course in 2014, and the key project leaders as well as the targeted biotoxins for the survey were all identified.

2 days 24-25 July 2013

3.2 Evaluation of the Project Outcomes Till the Year 2013 3.2.1 Theme/Program Thrust and Issues:

(1) Theme/Program Thrust:

Thrust II: Enhancing Capacity & Competitiveness to Facilitate International and Intra-regional Trade (2) Issues in the region at the beginning of the study:

Consumption of a variety of shellfish and fish causes an increasing number of human intoxications around the world. Around 400 poisonous fish species exist and, by definition, the substances responsible for the toxicity of these species are biotoxins. Marine biotoxins represent a significant and expanding threat to human health in many parts of the world. The impact is visible in terms of human poisoning or even death following the consumption of contaminated shellfish or fish, as well as mass killings of fish and shellfish, and the death of marine animals and birds. The Codex Alimentarius Code of Practice for Fish and Fishery Products (CAC/RCP 52-2003) defined biotoxins as poisonous substances naturally present in fish and fishery products or accumulated by the animals feeding on toxin producing algae, or in the water containing toxins produced by such organisms.

Monitoring seafood for toxicity is essential to manage the risks. However, there are several limitations in monitoring for toxicity such as the variation in toxin content between individual shellfish, different detection and even extraction methods for the various toxins, requiring a decision which toxins one is testing for, and the frequency of sampling to ensure that toxicity does not rise to dangerous levels in temporal or spatial gap between sampling times or locations. Furthermore, the growing harvest of non-traditional shellfish (such as moon snails, whelks, barnacles, etc.) may increase human health problems and management responsibilities.

MFRD has conducted a project on biotoxins monitoring in ASEAN from 2009 to 2012 to increase the attention in expanding and improving initiatives to monitor, detect and share information on marine biotoxins in order to reduce the public health risks associated with the consumption of contaminate shellfish and fish. The project had covered training in analytical methods for Diarrhoetic Shellfish Poisoning (DSP) toxins, lipophilic toxins, Paralytic Shellfish Poisoning (PSP) toxins and Tetradotoxin (TTX) and a monitoring survey on PSP toxin in ASEAN Member Countries.

This new project is an extension of the previous project to address the needs of Member Countries and continue with capability building in biotoxins analyses and monitoring, with the proposed focus on other biotoxins like Amnesic Shellfish Poisoning (ASP) toxin and Azaspiracids (AZA). These 2 biotoxins were raised as part of the training needs by Member Countries during the RTC of the previous project in 2009. Brevetoxins (BTX) is also recommended to be included in the new project as ASP, AZA and BTX, along with DSP and PSP, should be regulated according to CODEX for shellfish. ASP (Domoic Acid) is produced by Pseudo-nitzschia algae (diatom). It is a neurotoxin that can cause stomach upset, disorientation and in severe cases, a loss of short term memory. AZA are lipophilic marine toxins and AZA poisoning results in symptoms like nausea, vomiting, diarrhea and stomach cramps. BTX are neurotoxins produced by a dinoflagellatte (phytoplankton) known as Karenia brevis, resulting in typical neurotoxic shellfish poisoning (NSP) symptoms.

81 3.2.2 Expected Final Goal of the Project:

- Upgrade regional laboratory capabilities and credibility for testing of ASP, AZA and BTX biotoxins through conduct of a regional training course on methodologies for Member Countries and biotoxins monitoring survey in Member Countries.

- Establish biotoxins monitoring programmes in Member Countries for routine surveillance testing of fish and fisheries products especially in those countries that do not have such programmes.

- Improve knowledge and understanding on levels on biotoxins occurrences and incidences in fish and fisheries products in the ASEAN region and facilitate exchange of information among Member Countries.

3.2.3 “Steps” Toward Achieving Final Goal:

Outline

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